cdk6 cyclind3  (Carna Inc)

Journal: Drug Design, Development and Therapy

Article Title: Design, Synthesis, and Biological Evaluation of 2-Anilino-4-Triazolpyrimidine Derivatives as CDK4/HDACs Inhibitors

doi: 10.2147/DDDT.S351049

Figure Lengend Snippet: Enzymatic Inhibitory Activities of Compounds 11a~11k and 16a~16c

Article Snippet: CDK2/CycA2 (04–102), CDK4/CyclinD3 (04–105), and CDK6/CyclinD3 (04–107) were all purchased from Carna Biosciences, Inc. (Kobe, Japanese).

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Design, Synthesis, and Biological Evaluation of 2-Anilino-4-Triazolpyrimidine Derivatives as CDK4/HDACs Inhibitors

doi: 10.2147/DDDT.S351049

Figure Lengend Snippet: CDK4/6 Inhibitory Activities of Representative Active Compounds

Article Snippet: CDK2/CycA2 (04–102), CDK4/CyclinD3 (04–105), and CDK6/CyclinD3 (04–107) were all purchased from Carna Biosciences, Inc. (Kobe, Japanese).

Techniques:

Journal: RSC Advances

Article Title: Hybrid nucleobase–heterocycle–2-oxindole scaffolds as innovative cell cycle modulators with potential anticancer activity

doi: 10.1039/d5ra04997k

Figure Lengend Snippet: Schematic of the binding regions within the ATP active site of CDK6 in complex with palbociclib. The key interaction regions are (1) hinge (adenine) region—Val101, Leu152, Glu99, and Phe98 residues; (2) ribose pocket—Asp163 and Lys43 residues; (3) hydrophobic region—Phe98 and Leu152 residues; (4) solvent region—Asp163 and Lys43 residues; and (5) phosphate pocket—Lys43, Asp145, and Glu61 residues.

Article Snippet: CDK6 inhibitory activity was evaluated using the ADP-GloTM Kinase Assay CDK6 kit (BPS Bioscience, San Diego, CA, USA) following the provided protocol.

Techniques: Binding Assay, Solvent

Journal: RSC Advances

Article Title: Hybrid nucleobase–heterocycle–2-oxindole scaffolds as innovative cell cycle modulators with potential anticancer activity

doi: 10.1039/d5ra04997k

Figure Lengend Snippet: Snapshots of palbociclib inside the active site of CDK6 over 50 ns long MDS (5, 10, 20, 30, 40 and 50 ns).

Article Snippet: CDK6 inhibitory activity was evaluated using the ADP-GloTM Kinase Assay CDK6 kit (BPS Bioscience, San Diego, CA, USA) following the provided protocol.

Techniques:

Journal: RSC Advances

Article Title: Hybrid nucleobase–heterocycle–2-oxindole scaffolds as innovative cell cycle modulators with potential anticancer activity

doi: 10.1039/d5ra04997k

Figure Lengend Snippet: Snapshots of compound 12b inside the active site of CDK6 over 50 ns long MDS (5, 10, 20, 30, 40 and 50 ns).

Article Snippet: CDK6 inhibitory activity was evaluated using the ADP-GloTM Kinase Assay CDK6 kit (BPS Bioscience, San Diego, CA, USA) following the provided protocol.

Techniques:

Journal: RSC Advances

Article Title: Hybrid nucleobase–heterocycle–2-oxindole scaffolds as innovative cell cycle modulators with potential anticancer activity

doi: 10.1039/d5ra04997k

Figure Lengend Snippet: Representative example of a halogen bond in compound 12b with His100 and vicinal Val101 in the CDK6 active site.

Article Snippet: CDK6 inhibitory activity was evaluated using the ADP-GloTM Kinase Assay CDK6 kit (BPS Bioscience, San Diego, CA, USA) following the provided protocol.

Techniques:

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CDK6/CyclinD3 Kinase Assay , Promega , Cat# V4510.

Techniques: Virus, Recombinant, Control, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software